PI(3,4,5)P3 interactome

B Catimel; MX Yin; C Schieber; M Condron; H Patsiouras; J Catimel; DEJE Robinson; LSM Wong; EC Nice; AB Holmes; AW Burgess

Journal article

PI(3,4,5)P3 interactome

B Catimel, MX Yin, C Schieber, M Condron, H Patsiouras, J Catimel, DEJE Robinson, LSM Wong, EC Nice, AB Holmes, AW Burgess

Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2009

DOI: 10.1021/pr900320a

Abstract

Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments. © 2009 American Chemical Society.

University of Melbourne Researchers

Andrew Holmes Author

Antony Burgess Author

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Grants

Awarded by Discovery Project

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by the Australian Research Council, Discovery Project, Grant DP0770668. General support was provided by CSIRO and VESKI (Victorian Endowment for Science, Knowledge and Innovation). We thank Professor Mark Lemmon, University of Pennsylvania, Philadelphia, PA, for his generous gift of PLC delta PH and DynIPH in plasmids.